rabbit anti β3 integrin  (Cell Signaling Technology Inc)

Journal: PLOS One

Article Title: A novel sialylation pathway mediated by extracellular vesicles in aggressive prostate cancer

doi: 10.1371/journal.pone.0329014

Figure Lengend Snippet: (A) Immunoblotting (IB) analysis of ST6GAL1, CD9 and Syntenin in lysates of sEVs isolated by IDG separation from plasma of patients with invasive (top, Prep# 1) or noninvasive (bottom, Prep# 2) PrCa; the total volume (30 μL) of each fraction was used. (B) IB analysis of ST6GAL1, CD9, TSG101 and Calnexin (CNX) in sEVs from plasma of healthy donors isolated by IDG and in PC3 total cell lysate (TCL); the total volume of each fraction and 40 µg of PC3 TCL were used. (C) IB analysis of CD41 and the αVβ3 integrin (β3) in platelet (PLT) lysate and sEVs from the plasma of healthy donors and patients with PrCa; 10 µg of TCL and 20 µg of sEV lysates were used. (m) indicates the membrane-bound ST6GAL1 form and (s) the soluble form.

Article Snippet: Rabbit monoclonal antibodies to: β-Actin (Cell Signaling, 4970, RRID:AB_2223172) 1:1000, Tumor susceptibility gene 101 (TSG101) (Abcam, ab125011, RRID:AB_10974262) 1:1000, β3 integrin (Cell Signaling, 13166S, RRID:AB_2798136) 1:1000, Syntenin (Abcam, ab133267, RRID:AB_11160262) 1:1000, Programmed cell death ligand 1 (PDL1) (Cell Signaling, 13684, RRID: AB_2687655) 1:1000 and CD41 (Abcam, ab134131, RRID:AB_2732852) 1:1000 were used.

Techniques: Western Blot, Isolation, Clinical Proteomics, Membrane